IFNGR1 Knockout cell line (Hep G2)

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IFNGR1 Knockout cell line (Hep G2) Order

IFNGR1 Knockout cell line (Hep G2)
Catalog#
YKO-HT3146
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
IFNGR1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
IFNGR1 Knockout cell line (Hep G2)
Species
Human
Gene Name
IFNGR1
Gene ID
3459
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript IFNGR1-201)
Validation: PCR+Sanger Sequencing
IFNGR1 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the IFNGR1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, IFNGR1 gene knockout IFNGR1 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
IFNGR1 Knockout cell line (Hep G2)
IFNGR1 Knockout cell line (Hep G2)
Catalog#
YKO-HT3146
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • IFNGR1 Knockout cell line (THP-1)

    IFNGR1 Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: IFNGR1
    Gene ID: 3459
    Size: 1*10^6
    Catalog#: YKO-HT0651
    YKO-HT0651(THP-1)
  • Ifngr1 Knockout cell line (BV2)

    Ifngr1 Knockout cell line (BV2)
      inquiry   More details

    Organism: Mouse
    Gene Name: Ifngr1
    Gene ID: 15979
    Size: 1*10^6
    Catalog#: YKO-M183
    YKO-M183(BV2)
  • IFNGR1 Knockout cell line (HeLa)

    IFNGR1 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: IFNGR1
    Gene ID: 3459
    Size: 1*10^6
    Catalog#: YKO-HS3600
    YKO-HS3600(HeLa)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • IFNGR1 Knockout cell line (THP-1)

    IFNGR1 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: IFNGR1
    Gene ID: 3459
    Size: 1*10^6
    Catalog#: YKO-HT0651
      inquiry   More details
  • Ifngr1 Knockout cell line (BV2)

    Ifngr1 Knockout cell line (BV2)

    Organism: Mouse
    Gene Name: Ifngr1
    Gene ID: 15979
    Size: 1*10^6
    Catalog#: YKO-M183
      inquiry   More details
  • IFNGR1 Knockout cell line (HeLa)

    IFNGR1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: IFNGR1
    Gene ID: 3459
    Size: 1*10^6
    Catalog#: YKO-HS3600
      inquiry   More details

IFNGR1 Knockout cell line (Hep G2) Order

IFNGR1 Knockout cell line (Hep G2)
Catalog#
YKO-HT3146
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
IFNGR1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
IFNGR1 Knockout cell line (Hep G2)
Species
Human
Gene Name
IFNGR1
Gene ID
3459
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript IFNGR1-201)
Validation: PCR+Sanger Sequencing
IFNGR1 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the IFNGR1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, IFNGR1 gene knockout IFNGR1 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
IFNGR1 Knockout cell line (Hep G2)
IFNGR1 Knockout cell line (Hep G2)
Catalog#
YKO-HT3146
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • IFNGR1 Knockout cell line (THP-1)

    IFNGR1 Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: IFNGR1
    Gene ID: 3459
    Size: 1*10^6
    Catalog#: YKO-HT0651
    YKO-HT0651(THP-1)
  • Ifngr1 Knockout cell line (BV2)

    Ifngr1 Knockout cell line (BV2)
      inquiry   More details

    Organism: Mouse
    Gene Name: Ifngr1
    Gene ID: 15979
    Size: 1*10^6
    Catalog#: YKO-M183
    YKO-M183(BV2)
  • IFNGR1 Knockout cell line (HeLa)

    IFNGR1 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: IFNGR1
    Gene ID: 3459
    Size: 1*10^6
    Catalog#: YKO-HS3600
    YKO-HS3600(HeLa)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • IFNGR1 Knockout cell line (THP-1)

    IFNGR1 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: IFNGR1
    Gene ID: 3459
    Size: 1*10^6
    Catalog#: YKO-HT0651
      inquiry   More details
  • Ifngr1 Knockout cell line (BV2)

    Ifngr1 Knockout cell line (BV2)

    Organism: Mouse
    Gene Name: Ifngr1
    Gene ID: 15979
    Size: 1*10^6
    Catalog#: YKO-M183
      inquiry   More details
  • IFNGR1 Knockout cell line (HeLa)

    IFNGR1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: IFNGR1
    Gene ID: 3459
    Size: 1*10^6
    Catalog#: YKO-HS3600
      inquiry   More details

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